Fermentation was performed by inoculating Lactobacillus kefiranofaciens in cheese whey. I have separated the biomass after 72 h of fermentation and I want to measure exopolysaccharide i. e. kefiran in the fermented broth. Following a method described by Cheirsilp et al. 2018, I have precipitated kefiran by adding cold ethanol. My concern is, Is there any possibility to precipitating of sugars such as lactose or proteins present in the whey along with kefiran? Could you please suggest me regarding this or refer me standard protocols? Thanks in advance!
Dear Karim!
There is less possibility to precipitate the sugars with kefiran. However, your samples can be tested for the absence of other sugars or proteins by qualitative thin layer chromatography (TLC) and the Bradford method to confirm that no sugar was precipitated. You can follow the method used in the articles attached.
Hello Molka! Thank you very much for response. I will go through the articles nad will find out a way to carry out my experiments. Regards, Karim
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