I'm still newbie in NGS and my knowledge limited to few software. I'm trying to explorer to more software and method to gain my knowledge.
I'm looking for alternative software for gene and functional prediction, metabolic pathway construction and etc., is anyone willing to share their experience in yeast gene and functional prediction?
06 July 2012 8,721 2 View
There is too many sources of annotation software. Some can publish the complete genome even just using fully automatic annotation software. Yet, there is mis functional annotation. Different...
04 May 2012 8,642 0 View
This is my first time handling such genome data.
04 May 2012 5,632 0 View
I cannot make newbler run GUI after installation and restart, can anyone can help me solve such an issue? I'm using CentOS 6.2 and Redhat 6. Both have the same problem >_
04 May 2012 3,747 0 View
Looking for user experience feedback
04 May 2012 6,767 8 View
After quality filter, assembly, and validation. Is there is anything else or tiny thing that user could missing up. I'm wandering, the exact definition for advance assembly analysis
04 May 2012 8,677 2 View
During the conversion, the msg indicate the conversion fail, it's lock and lib not found. This situation also happend when I convert yeast velvet amos compatible to bank format.
04 May 2012 9,927 0 View
Do you know of any software that allows prediction and analyses of mis-assemble and QC of assembly result other than AMOS and CLC software?
04 May 2012 6,856 14 View
I failed to make it on either Illumina PE, Hiseq2000 (stuck at mery) and Roche 454 FLX (stuck to create overlap)
04 May 2012 443 7 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...
24 February 2021 8,856 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks
23 February 2021 6,518 3 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View
Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...
18 February 2021 8,719 1 View
I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...
17 February 2021 7,575 2 View