I cannot make newbler run GUI after installation and restart, can anyone can help me solve such an issue?
I'm using CentOS 6.2 and Redhat 6. Both have the same problem >_
I'm looking for alternative software for gene and functional prediction, metabolic pathway construction and etc., is anyone willing to share their experience in yeast gene and functional prediction?
06 July 2012 8,792 2 View
There is too many sources of annotation software. Some can publish the complete genome even just using fully automatic annotation software. Yet, there is mis functional annotation. Different...
04 May 2012 8,703 0 View
This is my first time handling such genome data.
04 May 2012 5,679 0 View
After quality filter, assembly, and validation. Is there is anything else or tiny thing that user could missing up. I'm wandering, the exact definition for advance assembly analysis
04 May 2012 8,729 2 View
Looking for user experience feedback
04 May 2012 6,816 8 View
During the conversion, the msg indicate the conversion fail, it's lock and lib not found. This situation also happend when I convert yeast velvet amos compatible to bank format.
04 May 2012 9,982 0 View
Do you know of any software that allows prediction and analyses of mis-assemble and QC of assembly result other than AMOS and CLC software?
04 May 2012 6,918 14 View
I failed to make it on either Illumina PE, Hiseq2000 (stuck at mery) and Roche 454 FLX (stuck to create overlap)
04 May 2012 496 7 View
I'm still newbie in NGS and my knowledge limited to few software. I'm trying to explorer to more software and method to gain my knowledge.
04 May 2012 8,389 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
How is biomass energy essential in the power generation in a country.
08 July 2024 9,477 2 View
Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...
07 July 2024 9,413 4 View