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Questions related from Brigitte Bruijns
I would like to quantify my DNA (with e.g. the Qubit) after a real-time amplification reaction. We do not have a qPCR kit at the moment, but I would like to run the reaction in real-time, so I can...
04 April 2018 7,409 4 View
I am measuring the concentration of 8 different solutions (7 samples and 1 blanco (which should be 0)) with 3 different methods. Each solution is measured by 3 analysts (with all 3 methods). I...
03 March 2017 7,021 8 View
When I perform a real-time PCR experiment (with for instance SYBR Green or EvaGreen), would you also stain the gel (to check the amplicon lenght) with this dye or is the presence of the dye from...
03 March 2016 8,738 9 View