ATM is mutated in A-T patients. And A-T patients are always with a higher risk of cancer. But the status of ATM in the established cancer cell lines is seldom described. Dose anybody know which cancer cell line(s) is ATM-defective?
Seperating cytoplasmic and nuclear protein is usually conducted for studing protein distribution, especially transcrition factors, by western blotting. Tubulin is the most used cytoplasmic marker...
04 May 2018 3,313 2 View
With the increasing studies of microRNAs, more and more research reported that a specific miRNA could target multiple targets of a signaling pathway of related pathways. In some of these papers,...
05 June 2014 2,187 4 View
I am working to find out which transcription factors (TFs) may binding to my target gene’s promoter to regulate its expression. In the past, I used the ConSIte web site to find the potential TFs...
09 October 2013 7,990 8 View
Recently, I detected the expression of a miRNA by Q-PCR. The Ct values for triplicates are exactly the same,Ct=35.0, given by the computer, howerve I noticed that in fact there was a little...
03 April 2013 1,595 5 View
I thought tumor-initiating cells are crucial in tumor initiating as it names and cancer stem cells are essential for maintenance of the population of cancer cells and account for treatment...
01 January 1970 3,744 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View