Seperating cytoplasmic and nuclear protein is usually conducted for studing protein distribution, especially transcrition factors, by western blotting. Tubulin is the most used cytoplasmic marker according to references. My question is whether GAPDH could serve as a choice of cytoplasmic marker? And are there any other cytoplasmic markers frequently used?
Rahul Krishnan
GAPDH can be used as a control protein in cytplasmic fraction. But in some instances it can cause varied intensities in the blot. SO better optimise one or two internal controls according to your experimental conditions.
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