How to easily(!) use FWE small volume correction in SPM?

I would like to threshold masked versions of my first level contrast images in SPM using RFT based FWE correction for small volumes. I've read about and seen the small volume correction button...

12 January 2021 9,205 2 View

How do I extract values from polygons or points from a NetCDF layer file for specific geographical locations at specific points in time?

I have a NetCDF layer file with monthly SPEI-6 values between the years 1950 and 2020 from Global Drought Monitor, a shapefile with polygons of geographical areas that I want to use for extracting...

16 November 2020 2,739 7 View

How can I add other published articles of mine?

Do not think that anymore details.

08 July 2020 8,453 2 View

How can I overcome preferential allele amplification?

Does anyone have any experience with preferential allele amplification from PCR? I am trying to amplify over a troublesome repetitive region of which heterozygotes are proving difficult to call...

05 June 2016 5,250 7 View

ADHD genes, migration and early interventions in schools

According to genetic population studies, there are differences in the frequency of different alleles of so-called ADHD genes (or "migration genes"), such as the DRD4 allele, depending on how long...

01 January 1970 2,398 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View