Is there an easy and reliable way to measure glycogen in cell cultures?

More Malin Wennström's questions See All

Topspin 4.1.4 Relaxation analysis failes due to "wrong slice number" mistake during FID picking. Does someone know that error?

I performed an inversion recovery experiment to obtain the T1 relaxation time of my sample. 9 different vd values where used and 9 FIDs are visible when i open the 2d file. I can also extract them...

24 January 2023 5,602 1 View

Does postmortem delay affect levels of autoantibodies in serum?

Could autoantibodies in organs (such as spleen, brain or gut) be released into the blood after death, due to changes (integrity breakdown) occurring after death, and thereby alter the levels of...

03 February 2022 382 0 View

How to easily(!) use FWE small volume correction in SPM?

I would like to threshold masked versions of my first level contrast images in SPM using RFT based FWE correction for small volumes. I've read about and seen the small volume correction button...

12 January 2021 9,684 2 View

How do I extract values from polygons or points from a NetCDF layer file for specific geographical locations at specific points in time?

I have a NetCDF layer file with monthly SPEI-6 values between the years 1950 and 2020 from Global Drought Monitor, a shapefile with polygons of geographical areas that I want to use for extracting...

16 November 2020 3,010 7 View

How can I add other published articles of mine?

Do not think that anymore details.

08 July 2020 8,542 2 View

How to calculate the time it takes to change pH?

I have a closed system of 5L of seawater, S = 22.2 psu. I add O2 at a rate of 0.1 L/min. The start alkalinity and pCO2 is known. How do I caculate how long it takes (min) to remove all CO2 from...

27 January 2020 7,902 3 View

What research exists on business promotion and counseling services in Cultural and Creative Industries?

What research exists on how business promotion and counseling services are organized and carried out in Cultural and Creative Industries?

30 December 2017 9,776 3 View

Why is absorbance at 260 nm decreasing when I add the enzyme Dnase to DNA?

The idea was that Dnase cleavs the DNA which should make the absorbance increase. I follow this assay http://www.worthington-biochem.com/dnase/assay.html and use 6.25mM MgSO4, 1M acetate buffer...

18 January 2017 4,358 5 View

How can I overcome preferential allele amplification?

Does anyone have any experience with preferential allele amplification from PCR? I am trying to amplify over a troublesome repetitive region of which heterozygotes are proving difficult to call...

05 June 2016 5,265 7 View

P. lotor anatomy publications?

I'm looking for any sources, thanks in advance.

21 September 2015 2,513 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Rosaiah Kotikalapudi

http://www.abcam.com/glucose-assay-kit-ab65333.html

Mitchell A Sullivan

There's a great method in the following paper: Lowry, O. H.; Passonneau, J. V. A flexible system of enzymatic analysis; Academic Press, 1972.

It's been used successfully in recent paper's and I know can be used in for cultured cells. I've attached the paper of one of my colleagues.

Article Hyperphosphorylation of Glucosyl C6 Carbons and Altered Stru...

Christoph Metzendorf

Hej Malin, did you find an assay that works well for you? Could you share the protocol in that case?