Why are bacterial cells stored at -80°C and not at -20°C?

22 October 2020 1 3K Report

I noticed laboratories store bacterial cells at -80°C. I wonder if there any studies or anecdotal evidences showing that -80°C storage for bacterial cells works better, in terms of whichever variable, than -20°C storage.

Duried Alwazeer

At -20°C, some enzymes such as protease and lipase keep its activity besides some chemical reactions. For this reason the storage life at this temperature is few months compared to -80°C storage which is several years.

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