I think it is especially for moulds of the Aspergillus and Penicillium genera. I don't rmeember the reason! Do someone know? Thank you
#fungi #agar #inoculation #moulds #aspergillus #penicillium
Historically, mycology was based on morphology : color, shape, size and spores of the fungus were used for the first categorizations of fungal species. Morphology of a fungus varies depending on the physiological state and environment, a fungus is usually grown on several different growth media. Inoculation of a fungus on agar plates is done using the technique called three-point inoculation, where a toothpick or inoculation loop containing fungal spores is poked into the agar in three different spots on the plate. Growth and sporulation were observed after 5 days of incubation at 30°C.
General purpose media that are commonly used for fungal culture are potato dextrose agar and Sabouraud dextrose agar (fungi), malt extract, and less commonly brain heart infusion medium.
Hi. I think the inoculation points are simply repeats. It's just to optimize transplanting...
Thanks, Johan De meester for sharing, this make sense and is a great overview. I was wondering, specifically, on why on tree points? Is it "only" a matter of making sure the observation is robust as done in triplicate, as Wendyam Fabrice Nikiema kindly points out, or there is more than (should) meet the eyes? Wasn't there something also related to observing the areas of interaction between the colonies? As if to describe how they react to themselves? Thanks!
I believe it is practical, in my samples I could see that it really optimized the work, because the contaminations could easily expand in a single colony. With three points, even if the contamination affects one of the colonies, we can know the correct morphology based on the others. Corrado Nai
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