Is there a software/plugin/tool which allows us to select a combination of multiple primary and secondary antibodies to be used for immunofluoresence?

28 July 2015 1 1K Report

I was doing a triple staining(staining 3 proteins) with a different combination of antibodies from different species and was irritated by choices. Should I choose goat anti-rabbit conjugated to Alexa488 or donkey anti rabbit conjugated to Cy3 and so on...Is there some tool where we can input our constrains and the tool presents us with possible options ?

Leslie Rietveld

Hi Apoorva,

I would recommend using the Fluorescence Spectraviewer created by Life Technologies. It allows you to select from the various fluorescent dyes, view the excitation and emission spectra, and then input your specific light sources and filters to see what dyes will work best with your system.

I used it throughout my masters and I highly recommend it as it is super simple to use.

Good luck!

https://www.lifetechnologies.com/ca/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

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