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22 February 2021 9,932 11 View

G-mmpbsa- file error during one step calculation?

Hello, I am using the one-step approach from https://rashmikumari.github.io/g_mmpbsa/single_protein_ligand_binding_energy.html to calculate binding energy between protein and ligand. However, I...

09 December 2020 7,302 1 View

In colorimetric analysis, can we compare absorbance measurement from spectrometer and optical density measurements from image analysis using scanner?

There are two most popular methods for colorimetric analysis i.e. spectrometer data and image analysis using a scanner. For example, in order to study the color intensity of films, I have measured...

01 June 2020 4,911 1 View

Can we expect a reduction in the population of particles in SEM images in the form of particles and after making their films?

I took scanning electron microscope images of my samples in powder form and after coating their films. In powder form, a large number of particles can be seen however, in the films only a few...

21 July 2019 8,454 3 View

Is it possible that conscientiousness and openness to experience correlate with each other?

Is it possible that a person is both highly conscientious and highly open also? I conducted factor analysis on responses of personality traits namely conscientiousness and openness to experience...

17 June 2019 7,297 8 View

DNA isolation from mouse lungs?

Dear all, I want to isolate DNA from mouse lungs to check for gene deletion (The Cre is expressed specifically in Type II alveolar cells). I tried to isolate the DNA using the method discussed...

12 February 2019 5,159 5 View

What is the most efficient way to extract text from document images like Passport or License?

I'm currently working on a project to extract text from document- images (like passport and license) and storing the passport number and driving license number along with the name of the person in...

05 January 2019 6,545 5 View

Test Case generation using Machine Learning ?

Hello, i am looking into following things - "How AI will help, to understand what the code does and then generate test cases " could you please pass some hint for the same. regards Hiro Lalwani

26 December 2018 2,450 4 View

What can be the ideal rheological characteristics of a polymeric solution used for oral drug delivery?

Hello Everyone, I have made the polymeric solution of two different polymers. My aim involves preparation of beads from this polymeric solution and encapsulation of my drug to these beads that I...

26 August 2018 6,958 11 View

Which solvent is suitable for analysing the UV Vis spectra of a polydiacetylene compound?

I want to study UV Vis spectra of a polydiacetylene based compound. It is a polymer and is not soluble in most of the solvents. i tried dissolving it in ethanol, butanol, chloroform, DCM, THF, DMS...

25 October 2017 6,598 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View