Hello I want to address the activity of FimH type 1 in E. coli under different conditions. Does anybody knows of a transgenic strains, or a plasmid that carry such reporter gene?
Best regards.
Hello, I work inducing aggregation of E. coli with polymers, I need to asses the metabolic activity and survivability of my bacteria under different polymers. I am trying with FDA in DMSO,...
02 March 2018 8,606 3 View
Hello fellow scientists! I need to measure cell viability of E. coli under certain conditions, I would like to do it with Resazurin sodium salt, so I can measure the metabolic activity and then,...
31 December 2017 3,455 2 View
Hello, I would like to test the viability of E. coli with the dead assay method with a multireader plate. Does somebody has a protocol for this? I tried to do it at OD=1 with my compounds...
09 October 2017 7,392 0 View
Hello, I am working with different E. coli strains and I am finding quite difficult to know exactly what deletions, mutations, and insertions this strains have, to understand better their...
08 September 2017 5,690 3 View
Hello I began using Adobe Illustrator to draw nice figures for posters and publications, it is a really good software but quite challenging to master. Do you know of a repository with pre-made...
05 June 2017 4,714 2 View
I am just looking for a nice reference that mention that the outer membrane of Gram- bacterias is negatively charged.
03 April 2017 8,621 4 View
Hello, I have some E. coli k-12 strains that should express type 1 fimbriae. I would like to check if they do and if they are able to bind to mannose. Is there any antibody assay or similar to do...
03 April 2017 6,575 1 View
Hello everybody. I am actually working on glass slides silanization by different trimethoxysilanes like 3-aminopropyl trimethoxysilane. I use a 2% fresh solution (cannot reuse) of this silane on...
04 May 2016 6,366 13 View
Hello, I have a mirrored compound with a disulphide bond. I plant to reduce it using sodium tetrahydroborate in THF, and then in the same vial bind the thiol ends of the broken chain to a modified...
04 May 2016 1,054 2 View
I am working on Raman Spectroscopy with a Reninshaw machine, I need to plot the data and I do not have the software on my work computer. How do I transfer from wxd to a managable file format?
03 April 2016 7,826 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...
25 February 2021 1,011 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View