Is there a reporter for FimH type 1 activity in E. coli?

Hello I want to address the activity of FimH type 1 in E. coli under different conditions. Does anybody knows of a transgenic strains, or a plasmid that carry such reporter gene? Best regards.

02 March 2018 9,467 0 View

Fluorescein diacetate protocol for bacteria viability assay. Questions about his method?

Hello, I work inducing aggregation of E. coli with polymers, I need to asses the metabolic activity and survivability of my bacteria under different polymers. I am trying with FDA in DMSO,...

02 March 2018 8,606 3 View

Resazurin sodium salt protocol for E. coli?

Hello fellow scientists! I need to measure cell viability of E. coli under certain conditions, I would like to do it with Resazurin sodium salt, so I can measure the metabolic activity and then,...

31 December 2017 3,455 2 View

DEAD/ALIVE (Syto9/PI) with a multireader well plate protocol?

Hello, I would like to test the viability of E. coli with the dead assay method with a multireader plate. Does somebody has a protocol for this? I tried to do it at OD=1 with my compounds...

09 October 2017 7,392 0 View

Any good website for E. coli strains info?

Hello, I am working with different E. coli strains and I am finding quite difficult to know exactly what deletions, mutations, and insertions this strains have, to understand better their...

08 September 2017 5,690 3 View

Do you know of an images/schemes repository for Adobe Illustrator?

Hello I began using Adobe Illustrator to draw nice figures for posters and publications, it is a really good software but quite challenging to master. Do you know of a repository with pre-made...

05 June 2017 4,714 2 View

Fimbriae type 1 assay?

Hello, I have some E. coli k-12 strains that should express type 1 fimbriae. I would like to check if they do and if they are able to bind to mannose. Is there any antibody assay or similar to do...

03 April 2017 6,575 1 View

Glass slide functionalization by trimethoxysilanes set-up?

Hello everybody. I am actually working on glass slides silanization by different trimethoxysilanes like 3-aminopropyl trimethoxysilane. I use a 2% fresh solution (cannot reuse) of this silane on...

04 May 2016 6,366 13 View

Can click Thiol-ene inmobilisation with an alquene be done using THF as a solvent?

Hello, I have a mirrored compound with a disulphide bond. I plant to reduce it using sodium tetrahydroborate in THF, and then in the same vial bind the thiol ends of the broken chain to a modified...

04 May 2016 1,054 2 View

How to convert Invia Reninshaw wxd to text or excel?

I am working on Raman Spectroscopy with a Reninshaw machine, I need to plot the data and I do not have the software on my work computer. How do I transfer from wxd to a managable file format?

03 April 2016 7,826 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View