Any good website for E. coli strains info?

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Is there a reporter for FimH type 1 activity in E. coli?

Hello I want to address the activity of FimH type 1 in E. coli under different conditions. Does anybody knows of a transgenic strains, or a plasmid that carry such reporter gene? Best regards.

02 March 2018 9,467 0 View

Fluorescein diacetate protocol for bacteria viability assay. Questions about his method?

Hello, I work inducing aggregation of E. coli with polymers, I need to asses the metabolic activity and survivability of my bacteria under different polymers. I am trying with FDA in DMSO,...

02 March 2018 8,606 3 View

Resazurin sodium salt protocol for E. coli?

Hello fellow scientists! I need to measure cell viability of E. coli under certain conditions, I would like to do it with Resazurin sodium salt, so I can measure the metabolic activity and then,...

31 December 2017 3,455 2 View

DEAD/ALIVE (Syto9/PI) with a multireader well plate protocol?

Hello, I would like to test the viability of E. coli with the dead assay method with a multireader plate. Does somebody has a protocol for this? I tried to do it at OD=1 with my compounds...

09 October 2017 7,392 0 View

Do you know of an images/schemes repository for Adobe Illustrator?

Hello I began using Adobe Illustrator to draw nice figures for posters and publications, it is a really good software but quite challenging to master. Do you know of a repository with pre-made...

05 June 2017 4,714 2 View

Suitable reference that mention that Gram- bacteria outer membrane is negatively charged?

I am just looking for a nice reference that mention that the outer membrane of Gram- bacterias is negatively charged.

03 April 2017 8,621 4 View

Fimbriae type 1 assay?

Hello, I have some E. coli k-12 strains that should express type 1 fimbriae. I would like to check if they do and if they are able to bind to mannose. Is there any antibody assay or similar to do...

03 April 2017 6,575 1 View

Glass slide functionalization by trimethoxysilanes set-up?

Hello everybody. I am actually working on glass slides silanization by different trimethoxysilanes like 3-aminopropyl trimethoxysilane. I use a 2% fresh solution (cannot reuse) of this silane on...

04 May 2016 6,366 13 View

Can click Thiol-ene inmobilisation with an alquene be done using THF as a solvent?

Hello, I have a mirrored compound with a disulphide bond. I plant to reduce it using sodium tetrahydroborate in THF, and then in the same vial bind the thiol ends of the broken chain to a modified...

04 May 2016 1,054 2 View

How to convert Invia Reninshaw wxd to text or excel?

I am working on Raman Spectroscopy with a Reninshaw machine, I need to plot the data and I do not have the software on my work computer. How do I transfer from wxd to a managable file format?

03 April 2016 7,826 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View

Julian Libiseller-Egger

http://ecoliwiki.net/colipedia/index.php?title=Category:Strains&pageuntil=PA100-PA125%0APA100-PA125

http://www.openwetware.org/wiki/E._coli_genotypes

Frederic Lepretre

lot of subtypes for EC, try the UCSC database (some tools as a genome browser, table browser, beat, PCR...http://genome-euro.ucsc.edu/cgi-bin/hgTracks?hgsid=224460078_kblnIy8RP4hRuTDamFhPxODlcPP3&org=hub_4806_042&db=hub_4806_EscherichiaColi042Uid161985&position=Nc0176261%3A1-1%2C000&pix=982).

fred