Is there a reporter for FimH type 1 activity in E. coli?

Hello I want to address the activity of FimH type 1 in E. coli under different conditions. Does anybody knows of a transgenic strains, or a plasmid that carry such reporter gene? Best regards.

02 March 2018 9,467 0 View

Fluorescein diacetate protocol for bacteria viability assay. Questions about his method?

Hello, I work inducing aggregation of E. coli with polymers, I need to asses the metabolic activity and survivability of my bacteria under different polymers. I am trying with FDA in DMSO,...

02 March 2018 8,606 3 View

DEAD/ALIVE (Syto9/PI) with a multireader well plate protocol?

Hello, I would like to test the viability of E. coli with the dead assay method with a multireader plate. Does somebody has a protocol for this? I tried to do it at OD=1 with my compounds...

09 October 2017 7,392 0 View

Any good website for E. coli strains info?

Hello, I am working with different E. coli strains and I am finding quite difficult to know exactly what deletions, mutations, and insertions this strains have, to understand better their...

08 September 2017 5,690 3 View

Do you know of an images/schemes repository for Adobe Illustrator?

Hello I began using Adobe Illustrator to draw nice figures for posters and publications, it is a really good software but quite challenging to master. Do you know of a repository with pre-made...

05 June 2017 4,714 2 View

Suitable reference that mention that Gram- bacteria outer membrane is negatively charged?

I am just looking for a nice reference that mention that the outer membrane of Gram- bacterias is negatively charged.

03 April 2017 8,621 4 View

Fimbriae type 1 assay?

Hello, I have some E. coli k-12 strains that should express type 1 fimbriae. I would like to check if they do and if they are able to bind to mannose. Is there any antibody assay or similar to do...

03 April 2017 6,575 1 View

Glass slide functionalization by trimethoxysilanes set-up?

Hello everybody. I am actually working on glass slides silanization by different trimethoxysilanes like 3-aminopropyl trimethoxysilane. I use a 2% fresh solution (cannot reuse) of this silane on...

04 May 2016 6,366 13 View

Can click Thiol-ene inmobilisation with an alquene be done using THF as a solvent?

Hello, I have a mirrored compound with a disulphide bond. I plant to reduce it using sodium tetrahydroborate in THF, and then in the same vial bind the thiol ends of the broken chain to a modified...

04 May 2016 1,054 2 View

How to convert Invia Reninshaw wxd to text or excel?

I am working on Raman Spectroscopy with a Reninshaw machine, I need to plot the data and I do not have the software on my work computer. How do I transfer from wxd to a managable file format?

03 April 2016 7,826 3 View

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02 March 2021 3,060 3 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View