Hello, I have some E. coli k-12 strains that should express type 1 fimbriae.
I would like to check if they do and if they are able to bind to mannose.
Is there any antibody assay or similar to do the assay I am looking for?
Best regards.
Immunogold staining, using TEM.
Reference - FimH-based display of functional eukaryotic proteins on bacteria surfaces, science reports 2019.
Hello I want to address the activity of FimH type 1 in E. coli under different conditions. Does anybody knows of a transgenic strains, or a plasmid that carry such reporter gene? Best regards.
02 March 2018 9,583 0 View
Hello, I work inducing aggregation of E. coli with polymers, I need to asses the metabolic activity and survivability of my bacteria under different polymers. I am trying with FDA in DMSO,...
02 March 2018 8,746 3 View
Hello fellow scientists! I need to measure cell viability of E. coli under certain conditions, I would like to do it with Resazurin sodium salt, so I can measure the metabolic activity and then,...
31 December 2017 3,588 2 View
Hello, I would like to test the viability of E. coli with the dead assay method with a multireader plate. Does somebody has a protocol for this? I tried to do it at OD=1 with my compounds...
09 October 2017 7,519 0 View
Hello, I am working with different E. coli strains and I am finding quite difficult to know exactly what deletions, mutations, and insertions this strains have, to understand better their...
08 September 2017 5,819 3 View
Hello I began using Adobe Illustrator to draw nice figures for posters and publications, it is a really good software but quite challenging to master. Do you know of a repository with pre-made...
05 June 2017 4,831 2 View
I am just looking for a nice reference that mention that the outer membrane of Gram- bacterias is negatively charged.
03 April 2017 8,752 4 View
Hello everybody. I am actually working on glass slides silanization by different trimethoxysilanes like 3-aminopropyl trimethoxysilane. I use a 2% fresh solution (cannot reuse) of this silane on...
04 May 2016 6,494 13 View
Hello, I have a mirrored compound with a disulphide bond. I plant to reduce it using sodium tetrahydroborate in THF, and then in the same vial bind the thiol ends of the broken chain to a modified...
04 May 2016 1,169 2 View
I am working on Raman Spectroscopy with a Reninshaw machine, I need to plot the data and I do not have the software on my work computer. How do I transfer from wxd to a managable file format?
03 April 2016 8,241 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?
19 July 2024 5,239 2 View