15 Questions 43 Answers 0 Followers
Questions related from José Luis Brioso Jiménez
Hello I want to address the activity of FimH type 1 in E. coli under different conditions. Does anybody knows of a transgenic strains, or a plasmid that carry such reporter gene? Best regards.
03 March 2018 9,537 0 View
Hello, I work inducing aggregation of E. coli with polymers, I need to asses the metabolic activity and survivability of my bacteria under different polymers. I am trying with FDA in DMSO,...
03 March 2018 8,699 3 View
Hello fellow scientists! I need to measure cell viability of E. coli under certain conditions, I would like to do it with Resazurin sodium salt, so I can measure the metabolic activity and then,...
01 January 2018 3,539 2 View
Hello, I would like to test the viability of E. coli with the dead assay method with a multireader plate. Does somebody has a protocol for this? I tried to do it at OD=1 with my compounds...
10 October 2017 7,475 0 View
Hello, I am working with different E. coli strains and I am finding quite difficult to know exactly what deletions, mutations, and insertions this strains have, to understand better their...
09 September 2017 5,769 3 View
Hello I began using Adobe Illustrator to draw nice figures for posters and publications, it is a really good software but quite challenging to master. Do you know of a repository with pre-made...
06 June 2017 4,786 2 View
Hello, I have some E. coli k-12 strains that should express type 1 fimbriae. I would like to check if they do and if they are able to bind to mannose. Is there any antibody assay or similar to do...
04 April 2017 6,652 1 View
I am just looking for a nice reference that mention that the outer membrane of Gram- bacterias is negatively charged.
04 April 2017 8,697 4 View
Hello everybody. I am actually working on glass slides silanization by different trimethoxysilanes like 3-aminopropyl trimethoxysilane. I use a 2% fresh solution (cannot reuse) of this silane on...
05 May 2016 6,445 13 View
Hello, I have a mirrored compound with a disulphide bond. I plant to reduce it using sodium tetrahydroborate in THF, and then in the same vial bind the thiol ends of the broken chain to a modified...
05 May 2016 1,128 2 View
I am working on Raman Spectroscopy with a Reninshaw machine, I need to plot the data and I do not have the software on my work computer. How do I transfer from wxd to a managable file format?
04 April 2016 8,180 3 View
Hello. I am working on silica glass surface modification and I need to characterize it using Raman microscopy. This is my first time using this method and have no clue of the configuration that I...
03 March 2016 262 5 View
My aim is to develop a polymer brush on a glass surface, for that I will first functionalize with 3-CHLOROPROPYLTRIMETHOXYSILANE my glass slides. As a final product I need to replace the ending CL...
03 March 2016 5,872 1 View
I need to produce a polymer bush on a glass slide, for that I need to bind this organic RAFT polymer to a silicated surface rich on hydroxyl groups. -[Si(-OH)]n- My polymer has an hydroxyl group...
02 February 2016 2,831 5 View
I have just started my research and I have to try different organic polymers as the platform where E. coli biofilm will grow. 1) I need to know which are the best conditions (overnight culture and...
02 February 2016 9,191 11 View
