My 3T3L1 adipocytes are not responding to insulin properly in recent experiments.
I am wondering if my insulin stopped working. Is there a quick test for this?
I normally use 100 nM for 20 min and see an increase in phosphorylation of alt, but lately I do not.
Thank you!
Aline, the easiest and cheapest way might be to include a control with pharmaceutical insulin you can to source from a clinic or a patient.
Thank you! Unfortunately, I don't have the resources for it. I'd like to test what I have
Thank you, unfortunately this stems from a glucose assay not performing.
Did you try the different insulin signaling proteins phosphorylation, like AKT or GSK3beta, you can run WB or ELISA.
I am just trying to see if the molecule works without running it on cells
You could inject a little into a mouse and follow the blood sugar. But IMHO, simply buying a new vial is the better option.
p-Akt is increased after 1~5min of insulin treatment. You should treat insulin an then cells MUST be frozen using liquid nitrogen after 2 or 5 min.
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