TdT vs. PAP for polyadenylation?

Hello, From my understanding, there are two ways to add polyA tails to RNA. 1) with polyA polymerase (PAP) and ATP to RNA or 2) with terminal transferase (TdT) plus dATP and ddATP to ssDNA...

18 October 2020 6,078 2 View

How to report results from a multinomial logistic regression?

I have run a multinomial logistic regression and am interested in reporting the results in a scientific journal. Would it be alright to include a model summary table with the coefficients,...

01 June 2020 4,107 12 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

What effect size calculation to use for meta-analysis?

I am conducting a meta-analysis for the association between TNBC and BRCA1 and BRCA2. Can anybody help me as to what 2x2 frequency table to use- standardised mean difference, correlation...

05 March 2020 4,992 3 View

Have I got the settings right?

I want anyone to be able to download my paper "elderberry is antibacterial, antiviral etc" for free and easily. Have I got the settings right? With Covid-19 spreading I think elderberry (Sambucus...

02 March 2020 6,607 3 View

I'm looking for a measure of self-perceived attractiveness for a study I'm conducting at my university. Does anyone know of an existing measure?

I've looked around for a survey for this measure, but I can't find one anywhere. I originally was just using a universal 10-point rating, but my professor would like to see something more...

01 March 2020 6,529 3 View

Which software is best suited for conducting Life Cycle Analysis (LCA) on agricultural produce in India?

I am a student planning to use LCA on agricultural products in India. There are multiple options for LCA software, but either the software itself or the supporting database is expensive. However,...

10 February 2020 342 9 View

GFP/RFP constitutively expressed from integrated transgene in E. coli?

It would be very useful to have tame strains of E. coli that are always fluorescent. So far the only ones I can find are from this work: Pinheiro, Bergquist et al. (2008), Applied Microbiology and...

09 February 2020 9,918 2 View

How many E.coli cells should I sort via FACS for RNA-seq?

Hello, I have been trying to find studies that have FACS sorted E.coli in bulk for RNA extraction and RNA-seq. Maybe I am bad at lit-searching, but I have been surprised to not find a clear...

14 November 2019 4,668 3 View

Would monoclonal antibody aggregates effect the absorbance during a Bradford Assay?

I have purified an IgG from cell supernatant using a Protein G column. Following this size exclusion chromatography was used to determine aggregates of the IgG, monomeric antibody and antibody...

08 November 2019 1,487 3 View

Can anyone tell me the name of these parasites which I got from a marine fish?

I got these parasites from eye and intestine of a marine fish. Thank you.

09 December 2020 7,752 2 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

Measuring laser intensity in PPFD (Photosynthetic Photon Flux Density)?

Hi, I run my experiment with either red or green laser and I use the PPFD meter to measure the intensity before my experiment. Does it make any sense for monochromatic light? What would be the...

26 July 2020 1,095 1 View

What is the best method to get a good appearance or view or photo for Leishmania parasite????

i need an easiest technique for Fluorescent stain used with Fluorescent Microscope .

14 July 2020 3,551 3 View

How can I hide nodes with posterior probability under 0.5 in BEAST phylogenetic tree visualization?

06 July 2020 1,376 6 View

Hello, Is their anyone working on Parasites G mass, can help us by papers or researchers?

General Parasitology

30 January 2020 2,247 2 View

Doed anyone have a protocol for comet assay in chlamydomonas?

Hi everyone! I am looking for an efficient comey assay protocol in chlamydomonas reinhardtii if you have some suggestions let me know thanks a lot!

15 January 2020 698 3 View

Does anyone know the origin of this observation about kangaroos?

In his 2007 book Chasing Kangaroos, Tim Flannery rather extensively discusses the idea (treated in the text as something supported by scientific research, rather than an untested hypothesis) that...

09 January 2020 6,973 2 View

Doed anyone have a protocol for comet assay in chlamydomonas?

Hi everyone! I am looking for an efficient comey assay protocol in chlamydomonas reinhardtii if you have some suggestions let me know thanks a lot!

31 December 2019 1,383 3 View

Could anyone free for english editing service ??

One of my research article is rejected due to english language from Parasites and Vectors journal?? Is there anyone who able to assist me??

29 December 2019 5,207 8 View