Do you know any online database containing pictures (and explanation) about dry bone histology?

I'm interested in both pathological and normal histology.

15 January 2019 3,823 3 View

Why are E. coli strains used for overexpression of recombinant proteins often recA+ as opposed to recA-?

Many molecular biology E.coli strains are recA- to prevent recombination of the plasmid with the host chromosome, or scrambling of the plasmid, and/or mixing of more than one plasmid if...

09 May 2018 7,146 8 View

No title

09 March 2017 3,472 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

I need help converting a PAUP block into a MrBayes block - please help?

18 April 2016 8,561 1 View

Clinical Phase II to Phase III failures in oncology?

I am requesting the help of the RG community to provide me with recent (no older than 5 years) articles (from very high and only from very high ranked journals) clearly 1) indicating how many...

11 February 2016 4,471 18 View

How do you interpret a KEGG/KAAS output?

I've just run a KAAS analysis on my transcriptome. I'm a little unclear as to how to understand the output. For example, I have clicked on "Carbon Metabolism" and this image comes up (see...

21 April 2014 3,775 2 View

Has anyone used breath-easy membranes from Diversified Biotech for growing Chlamydomonas in 24-well plates?

We have tried and our cultures keep dying. Has anyone had this problem?

10 November 2013 4,297 0 View

Increasing/decreasing fluorescence when measuring RNA concentration?

When we measured RNA concentration using fluorescence method with Modulus Turner Biosystems and measured the same sample a few times in a row without opening the lid, sometimes the fluorescence...

05 September 2013 2,591 5 View

Can petal colour be determined only by genetics (in Rubus: white or pink petals)?

01 January 1970 7,953 11 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View