RNA isolation after TTC staining

More Kiss Bernadett's questions See All

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

Intracellular parasite of chlamydomonas?

Does anyone have experience with parcitism of Chlamydomonas? We have a highly infectious parasite in our lab and can't find much information on it. I've attached some images.

11 June 2024 9,637 2 View

How do I understand the payoff matrix in Axelrod and Hamilton?

I am fighting my way through Axelrod and Hamilton (1981) on the Prisoners Dilemma. this is the payoff matrix they present for the PD. But they only present the payoffs for player A. Normally,...

13 February 2024 6,294 1 View

Do you know any online database containing pictures (and explanation) about dry bone histology?

I'm interested in both pathological and normal histology.

15 January 2019 4,043 3 View

What is the best vital dye for sporangia?

I am looking for a vital dye - preferably quick & easy as opposed to fluorescent dyes - for sporangia viability testing for downy mildew (Peronospora farinosa).

03 June 2018 8,651 4 View

Why are E. coli strains used for overexpression of recombinant proteins often recA+ as opposed to recA-?

Many molecular biology E.coli strains are recA- to prevent recombination of the plasmid with the host chromosome, or scrambling of the plasmid, and/or mixing of more than one plasmid if...

09 May 2018 7,325 8 View

Could you please recommend me articles and books about how to tell what was the mechanism and cause of a given skull fracture pattern?

I'm especially interested in blunt force trauma and where was the impact's location.

16 November 2017 8,653 3 View

How to export NEXUS, bootstrap phylogenetic tree from MEGA?

I am exporting a phylogenetic tree with bootstrap from MEGA and I need the following NEXUS format to integrate the phylo data into a geometric morphometric analysis in MorphoJ software. The...

27 October 2017 4,604 8 View

Somebody can identify this seed from El Rocío, Spain?

It was collected in the marshland El Rocío, Spain. It has a very strong seed coat. The attached pictures shows two pieces from different angle of the same species. Thank you for the answers!

09 March 2017 3,614 3 View

Somebody can identify this seed?

It was collected in the ricefields around Isla Major, near to Doñana National Park, Spain. Thank you very much for your answers!

05 December 2016 5,505 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,080 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,881 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,336 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,364 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,726 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,706 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 500 2 View