Do you know any online database containing pictures (and explanation) about dry bone histology?

I'm interested in both pathological and normal histology.

15 January 2019 3,823 3 View

Why are E. coli strains used for overexpression of recombinant proteins often recA+ as opposed to recA-?

Many molecular biology E.coli strains are recA- to prevent recombination of the plasmid with the host chromosome, or scrambling of the plasmid, and/or mixing of more than one plasmid if...

09 May 2018 7,146 8 View

RNA isolation after TTC staining

How much the staining affects the RNA isolation and proteomic test after using the TTC stained heart tissue?

26 June 2017 9,924 2 View

No title

09 March 2017 3,472 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

I need help converting a PAUP block into a MrBayes block - please help?

18 April 2016 8,561 1 View

Clinical Phase II to Phase III failures in oncology?

I am requesting the help of the RG community to provide me with recent (no older than 5 years) articles (from very high and only from very high ranked journals) clearly 1) indicating how many...

11 February 2016 4,471 18 View

How do you interpret a KEGG/KAAS output?

I've just run a KAAS analysis on my transcriptome. I'm a little unclear as to how to understand the output. For example, I have clicked on "Carbon Metabolism" and this image comes up (see...

21 April 2014 3,775 2 View

Has anyone used breath-easy membranes from Diversified Biotech for growing Chlamydomonas in 24-well plates?

We have tried and our cultures keep dying. Has anyone had this problem?

10 November 2013 4,297 0 View

Increasing/decreasing fluorescence when measuring RNA concentration?

When we measured RNA concentration using fluorescence method with Modulus Turner Biosystems and measured the same sample a few times in a row without opening the lid, sometimes the fluorescence...

05 September 2013 2,591 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View