Is there a fungal expression system which is equally well established to that of Pichia Pastoris for industrial fermentation?

More Nikunj Daga's questions See All

How do I verify the Harmonic response simulation? What kind of tests can I do except vibration test?

I am currently working on an air compressor unit. I have already done the simulation and testing for modal analysis on the compressor(static condition). Now I want to do a Harmonic response...

12 October 2023 8,168 2 View

How to identify the regulations of river flow?

is it possible to identify the regulation or what is the probability that the river flow is regulated, based on only observed streamflow data and/or meteorological data such as precipitation? If...

08 February 2023 2,892 3 View

Why does religation problem persists in Vector backbone while digesting with two different restriction enzymes?

Our aim is to transfer gene from bacterial plasmid(pET22b) into mammalian expression vector pACGFP1-N1, i have decided restriction sites available in pACGFP1-N1 vector MCS but while doing...

24 January 2023 446 3 View

How to prevent pellet formation in aspergillus oryzae ?

I am trying to inoculate transformed fungal colonies of aspergillus oryzae from drop out media using tooth pick. I am incoulating them in 10ml YPD media with the help of toothpeak. I am getting...

15 July 2022 10,000 3 View

What are the tools to analyze protein network other than STRING of more then 2000 proteins?

We have a protein profile of around 4000 proteins, and we tried Cytoscape since STRING has limitation of 2000 maximum , Cytoscape slowed down the system and taking more time, i also tried GEPHI...

14 February 2022 2,083 0 View

What is the alternative to Glucanex lysing enzyme ?

Sigma lysing enzyme L1412 has been discontinued. Is there a known commercially available alternative to this ?

19 April 2021 4,726 4 View

How can we extract digital elevation data from Google Earth?

Is there any option or methodology to download Google Earth's Digital Elevation Data? Is there any tools or software available to extract the digital elevation model (DEM) of Google Earth?

12 April 2021 1,588 3 View

Error in QGIS: The following layers were not correctly generated.. is there any solution for this?

While performing "Terrain Analysis" tool such as "Strahler Order, channel network and drainage basin" of SAGA in QGIS, I often found the error message as result which says "The following layers...

15 March 2021 8,307 7 View

Journals with low APC or no APC?

Do you know any journals in the area of medicine/pediatrics/nutrition that publish protocols of systematic reviews? No APC or Low APC

04 January 2021 655 14 View

What is the good Molprobity score for a protein homology model??

I build a homology model for a protein which show 44% sequence identity with its template and molprobity score is 1.59

27 December 2019 3,416 4 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Jai Ghosh

Yes it does exist. You may look up references on Aspergillus oryzea or Rhizopus nigricans.

Nikunj Daga

I could not find any commercially available expression systems. Being manufacturers there is very rare opportunity to develop the system from the scratch. For example, Invitrogen offers fantastic system for expression in pichia, likewise I am searching for Fungi. Say an aspergillus host, expression vector and cloning vector per se.