Is there a database for bacterial protein mutants?

More Viktor Attila Ambrus's questions See All

Will rat colon cancer cells (DHD/K12) derived from BDIX rats grow in Sprague Dawley ?

we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?

23 January 2021 1,437 2 View

How can I indicate to you that I am in fact the author of a book that you have informed me about for quoting other work of mine?

The question concerns this title: Viktor Smith Naming and Framing: Understanding the Power of Words across Disciplines, Domains, and Modalities. Routledge. All the best, Viktor

01 January 2021 4,197 1 View

Who has scheelite (CaWO4) activated by Eu, Sm, Tb, Er or other rare earths?

I would be grateful to my colleagues for the transfer of samples for the purpose of comparison with natural samples in terms of luminescent properties.

03 March 2020 6,099 1 View

Can anyone identify this plant?

This liana grows in the greenhouse of O.V. Fomin Botanical Garden of Taras Shevchenko National University of Kyiv (Ukraine). The plant has been introduced from Botanical Garden in Batumi...

29 January 2020 1,972 16 View

Compatible of QIAseq Stranded Total RNA Lib Kit and Ribominus Transcriptome Isolation Kit?

Dear all, I'm going to perform RNA-Seq with a QIAseq Stranded Total RNA Lib Kit and depletion with Ribominus Transcriptome Isolation Kit/ Ribominus concentration Kit. Does anyone have...

28 October 2019 8,120 1 View

Low bacterial RNA yeld with RNeasy MiniKit?

Dear all, I am trying to do an RNA extraction of different bacterial species with RNAeasy mini kit, protokol #4 (lysozyme lysis + proteinase K digestion). Unfortunately, I have a fairly low...

27 October 2019 5,537 0 View

What is a relation between relaxation time and mean free path for ideal gas?

These two values should be interconnected throufg integral of collisions. But I can not find a clear formula.

02 November 2018 3,613 1 View

Could you suggest some good algorithms for photo similarity recognition?

I would like to be able to compare photographs of places (mainly indoor) with existing database of photos to geo tag them. If you would be interested, this could lead to some research...

14 September 2017 9,947 3 View

I have L-histidine hydrochloride monohydrate. Help me, please, how can I eliminate HCl and water molecule to obtain L-histidine only?

Will NaOH or KOH neutralize L-histidine hydrochloride? And how can we separate products from each other if it will?

27 June 2017 6,690 4 View

Mechanical effort depending on acceleration - any thoughts?

In Newtons II law the force F acting on a body may depend on time, position and velocity but not acceleration. Otherwise the parallelogram principle doesn't operate for partial accelerations. And...

10 August 2014 2,061 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Vicente Mendoza Reyes

You can use the MVD (Microbial Variome Database), which stores and describes point mutations, adaptive or not, in bacterial core genomes. The reference paper is Mol Biol Evol. 2013 Jun; 30(6): 1465–1470. Published online 2013 Mar 14. doi: 10.1093/molbev/mst048 bonne chance mon ami