we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?
An important question
Hello Viktor Justin
As I could understand, you are trying to establish a xenograft SD rat model using the colon cancer cell line derived from BDIX rat strain.
If it is so, the answer is yes.
It won't be a problem at all. In fact, we used human-derived colon cancer cells to develop the xenograft model.
Good luck!
hello everyone, Anyone can help me fix that error that occurred when I was running PGLS model in caper package .
21 February 2024 3,475 0 View
The dopant concentration is Nd(x) in transiton domain 0
20 February 2024 7,314 1 View
Does it matter how GPS coordinates are reported in field biology - as in, is there a general preference between reporting GPS data using sexagesimal degrees (Degrees, Minutes, Seconds) or decimal...
12 January 2024 9,045 2 View
Hello, Maybe someone has faced unusual threads stained with DAPI? The photo displays C2C12 cells fixed with 4% PFA for 15 minutes, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes, and...
08 October 2023 1,680 3 View
This problem is relatively easy solved when stiffeners are identical and obeying rotational Hooke's law, i.e., are linear. The case of different but comparable with each other stiffeness...
30 September 2023 5,248 1 View
One of the factors that can affect the quality control of the laboratory is the laboratory workers. However, some laboratory workers do not follow the procedures for various reasons. What methods...
03 September 2023 3,548 3 View
I have derived the relationship between an arbitrary compressive strain-stress diagram and critical buckling stress for the console of a given slendernes 'lamda'. Now I need to verify the plots...
01 September 2023 7,814 0 View
I am considering doing some research on the etymological origins of certain words related to student government and student leadership, particularly words such as "student", "pupil", etc., to...
08 August 2023 4,539 6 View
I am currently trying to just prime my Biotage Isolera but it keeps giving me an error saying pump A is stuck. I tried cleaning the check valve to see if that will clean anything, it did not....
22 June 2023 569 2 View
Do academic hiring committees consider book reviews? I enjoy doing book reviews because I get free books, small publications, and it allows me to stay up to date on recent scholarship in my...
21 May 2023 7,997 9 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View