I am using HT-29 cells, which are bing treated by food protein hydrolysates. The literature doesn't seem to be consistent on the concentration (mg/ml) of food proteins or peptides being used to treat these or other cells. In case of pure peptides the concentration range seem to be around 0.1- 10 microM. But when it comes to hydrolysates or intact proteins is anybody aware of a general consensus or articles published in this regard
There is no real consensus since the biological activity is dose-response. From one hydrolysate or peptide to another one the concentration can be different to obtain a response. Generally speaking with muscle cells we use or test concentrations of 1 ng/mL, microg/mL and exceptionnaly millig/mL.
I think there is no consensus. I used HT-29 cells to perform nanoparticle transportation before. I did MTT assay to see the cytotoxicity of different concentration gradients to the cell and then get an approximate range. I used microg/mL. It just depends on the bioactivity of your samples.
Thank you for your perspectives, Laurent and Fengzhang! I found it very helpful!
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