Can viral gene delivery methods be employed in plants? Is there an alternate way to introduce RNA or DNA in plants for genome engineering by CRISPR-Cas system. If a model plant where to be made which already expresses the Cas9 enzyme. So is there anyway by which guided RNA can be transferred to these plant cells already containing Cas enzyme.
There are many plant viruses which have been engineered for gene transfer....geminivirus, Tobacco mosaic virus, Potato virus X, Cowpea mosaic virus. There are quite a few reviews on this topic....
Neither lenti nor AAV can infect a plant cell, however, there should be plant viruses that can be used for this application.
Yes, thank you!
I actually did a bit of searching since I was not able to get an answer earlier and was able to find out that gemini virus replicons are used as viral vectors in plants for tranfering TALENs and CRISPR system.
Article DNA Replicons for Plant Genome Engineering
Virus-based vectors are also used and delivered into plants for transient assay for recombinant DNA production due to their (1) replication ability, and (2) they can move from cell to cell (mobility). See attached paper:
"Agrobacterium is not alone: gene transfer to plants by viruses and other bacteria"
There are many plant viruses which have been engineered for gene transfer....geminivirus, Tobacco mosaic virus, Potato virus X, Cowpea mosaic virus. There are quite a few reviews on this topic....
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology