Why are flocs formed as a result of addition of chitosan to phospholipids or vice versa?

Flocs or aggregates are formed when phospholipids are mixed with chitosan (preparation of chitosan coated nanoparticles). Is anyone familiar with the chemistry underlying their interactions and...

10 November 2016 853 2 View

Is there a consensus on the physiologically relevant concentrations of food protein or peptides while performing cell culture studies?

I am using HT-29 cells, which are bing treated by food protein hydrolysates. The literature doesn't seem to be consistent on the concentration (mg/ml) of food proteins or peptides being used to...

08 September 2016 7,370 3 View

What are your thoughts on using a particular proportion of different tissues mixed to perform a batch of hydrolysis/fermentation?

06 July 2016 4,180 1 View

Can anyone suggest a journal with this particular citation format (author et al., year) ?

I want to cite using the style "author et al., year" for a paper using a referencing software, mendeley. The particular journal's referencing style doe not seem to be available. Can anyone suggest...

02 March 2015 807 2 View

Does anyone have an idea about the availability of N-analyzer for protein in the North India?

I want to estimate the N content from the lyophilized protein sample. Can you let me know if this equipment is available for the use?

01 February 2015 4,291 1 View

Do protein samples remain in a sticky form after lyophilization?

01 February 2015 4,533 7 View

After synthesizing nanoparticles from an emulsion using a surfactant with a sonicator, How should one proceed further?

Protein based nanoparticles are synthesized from an emulsion of oil and aqueous solution by providing energy from a sonicator and a surfactant is used sometimes for stability. Can the solutions...

09 October 2014 9,413 7 View

Can lentivirus or adeno-associated virus mediated transfer of RNA or DNA be made in plant cell?

Can viral gene delivery methods be employed in plants? Is there an alternate way to introduce RNA or DNA in plants for genome engineering by CRISPR-Cas system. If a model plant where to be made...

09 October 2014 6,538 7 View

Can splicing occur when a bacterial gene is transfected in an eukaryotic system?

Will splicing occur in a countinuous gene expressed in a eukaryotic system, if so, what steps can one take to prevent such potential problems?

09 October 2014 905 4 View

How do trypsin inhibitors or amylase inhibitors introgressed in GM crops for insect resistance not affect us?

Protease and amylase inhibitors are introduced into insect resistant crops produced by genetic engineering. Wouldn't these enzymes affect our digestive enzymes too??

09 October 2014 2,817 7 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View