Why are flocs formed as a result of addition of chitosan to phospholipids or vice versa?

Flocs or aggregates are formed when phospholipids are mixed with chitosan (preparation of chitosan coated nanoparticles). Is anyone familiar with the chemistry underlying their interactions and...

10 November 2016 853 2 View

Is there a consensus on the physiologically relevant concentrations of food protein or peptides while performing cell culture studies?

I am using HT-29 cells, which are bing treated by food protein hydrolysates. The literature doesn't seem to be consistent on the concentration (mg/ml) of food proteins or peptides being used to...

08 September 2016 7,370 3 View

Which is the best method for the isolation of oryza cystatins (cysteine protease inhibitory peptides) from rice bran?

For the isolation of cystatins (cysteine protease inhibiting peptides) from rice bran, I used isoelectric precipitation, but the sample was insoluble in the usual buffers and even SDS. I think...

10 November 2015 8,015 1 View

Can anyone suggest a journal with this particular citation format (author et al., year) ?

I want to cite using the style "author et al., year" for a paper using a referencing software, mendeley. The particular journal's referencing style doe not seem to be available. Can anyone suggest...

02 March 2015 807 2 View

Does anyone have an idea about the availability of N-analyzer for protein in the North India?

I want to estimate the N content from the lyophilized protein sample. Can you let me know if this equipment is available for the use?

01 February 2015 4,291 1 View

Do protein samples remain in a sticky form after lyophilization?

01 February 2015 4,533 7 View

After synthesizing nanoparticles from an emulsion using a surfactant with a sonicator, How should one proceed further?

Protein based nanoparticles are synthesized from an emulsion of oil and aqueous solution by providing energy from a sonicator and a surfactant is used sometimes for stability. Can the solutions...

09 October 2014 9,413 7 View

Can lentivirus or adeno-associated virus mediated transfer of RNA or DNA be made in plant cell?

Can viral gene delivery methods be employed in plants? Is there an alternate way to introduce RNA or DNA in plants for genome engineering by CRISPR-Cas system. If a model plant where to be made...

09 October 2014 6,538 7 View

Can splicing occur when a bacterial gene is transfected in an eukaryotic system?

Will splicing occur in a countinuous gene expressed in a eukaryotic system, if so, what steps can one take to prevent such potential problems?

09 October 2014 905 4 View

How do trypsin inhibitors or amylase inhibitors introgressed in GM crops for insect resistance not affect us?

Protease and amylase inhibitors are introduced into insect resistant crops produced by genetic engineering. Wouldn't these enzymes affect our digestive enzymes too??

09 October 2014 2,817 7 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View