02 February 2015 1 4K Report

I want to estimate the N content from the lyophilized protein sample. Can you let me know if this equipment is available for the use?

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Flocs or aggregates are formed when phospholipids are mixed with chitosan (preparation of chitosan coated nanoparticles). Is anyone familiar with the chemistry underlying their interactions and...

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Is there a consensus on the physiologically relevant concentrations of food protein or peptides while performing cell culture studies?

I am using HT-29 cells, which are bing treated by food protein hydrolysates. The literature doesn't seem to be consistent on the concentration (mg/ml) of food proteins or peptides being used to...

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What are your thoughts on using a particular proportion of different tissues mixed to perform a batch of hydrolysis/fermentation?

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06 July 2016 4,290 1 View

Which is the best method for the isolation of oryza cystatins (cysteine protease inhibitory peptides) from rice bran?

For the isolation of cystatins (cysteine protease inhibiting peptides) from rice bran, I used isoelectric precipitation, but the sample was insoluble in the usual buffers and even SDS. I think...

10 November 2015 8,140 1 View

Can anyone suggest a journal with this particular citation format (author et al., year) ?

I want to cite using the style "author et al., year" for a paper using a referencing software, mendeley. The particular journal's referencing style doe not seem to be available. Can anyone suggest...

02 March 2015 917 2 View

Do protein samples remain in a sticky form after lyophilization?

After freeze drying my extracted protein samples, it is seen that the samples  remain in a sticky texture. Is this normal?  

01 February 2015 4,673 7 View

After synthesizing nanoparticles from an emulsion using a surfactant with a sonicator, How should one proceed further?

Protein based nanoparticles are synthesized from an emulsion of oil and aqueous solution by providing energy from a sonicator and a surfactant is used sometimes for stability. Can the solutions...

09 October 2014 9,524 7 View

Can splicing occur when a bacterial gene is transfected in an eukaryotic system?

Will splicing occur in a countinuous gene expressed in a eukaryotic system, if so, what steps can one take to prevent such potential problems?

09 October 2014 1,014 4 View

Can lentivirus or adeno-associated virus mediated transfer of RNA or DNA be made in plant cell?

Can viral gene delivery methods be employed in plants? Is there an alternate way to introduce RNA or DNA in plants for genome engineering by CRISPR-Cas system. If a model plant where to be made...

09 October 2014 6,659 7 View

How do trypsin inhibitors or amylase inhibitors introgressed in GM crops for insect resistance not affect us?

Protease and amylase inhibitors are introduced into insect resistant crops produced by genetic engineering. Wouldn't these enzymes affect our digestive enzymes too??

09 October 2014 2,924 7 View

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