Why are flocs formed as a result of addition of chitosan to phospholipids or vice versa?

Flocs or aggregates are formed when phospholipids are mixed with chitosan (preparation of chitosan coated nanoparticles). Is anyone familiar with the chemistry underlying their interactions and...

10 November 2016 853 2 View

Is there a consensus on the physiologically relevant concentrations of food protein or peptides while performing cell culture studies?

I am using HT-29 cells, which are bing treated by food protein hydrolysates. The literature doesn't seem to be consistent on the concentration (mg/ml) of food proteins or peptides being used to...

08 September 2016 7,370 3 View

What are your thoughts on using a particular proportion of different tissues mixed to perform a batch of hydrolysis/fermentation?

06 July 2016 4,180 1 View

Which is the best method for the isolation of oryza cystatins (cysteine protease inhibitory peptides) from rice bran?

For the isolation of cystatins (cysteine protease inhibiting peptides) from rice bran, I used isoelectric precipitation, but the sample was insoluble in the usual buffers and even SDS. I think...

10 November 2015 8,015 1 View

Can anyone suggest a journal with this particular citation format (author et al., year) ?

I want to cite using the style "author et al., year" for a paper using a referencing software, mendeley. The particular journal's referencing style doe not seem to be available. Can anyone suggest...

02 March 2015 807 2 View

Do protein samples remain in a sticky form after lyophilization?

01 February 2015 4,533 7 View

After synthesizing nanoparticles from an emulsion using a surfactant with a sonicator, How should one proceed further?

Protein based nanoparticles are synthesized from an emulsion of oil and aqueous solution by providing energy from a sonicator and a surfactant is used sometimes for stability. Can the solutions...

09 October 2014 9,413 7 View

Can lentivirus or adeno-associated virus mediated transfer of RNA or DNA be made in plant cell?

Can viral gene delivery methods be employed in plants? Is there an alternate way to introduce RNA or DNA in plants for genome engineering by CRISPR-Cas system. If a model plant where to be made...

09 October 2014 6,538 7 View

Can splicing occur when a bacterial gene is transfected in an eukaryotic system?

Will splicing occur in a countinuous gene expressed in a eukaryotic system, if so, what steps can one take to prevent such potential problems?

09 October 2014 905 4 View

How do trypsin inhibitors or amylase inhibitors introgressed in GM crops for insect resistance not affect us?

Protease and amylase inhibitors are introduced into insect resistant crops produced by genetic engineering. Wouldn't these enzymes affect our digestive enzymes too??

09 October 2014 2,817 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View