Is their any example to solve Multi phase and Multi Species model in fluid flow?

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Which method/ open-source software is the best for Microstructure modeling? Any relevant resources regarding microstructure modeling?

I am learning microstructure modeling and also looking for some resources and opensource software to start with.

05 April 2024 4,576 3 View

Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF)?

Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF). Will there be any species incompatibility related unwanted effect on...

12 November 2023 1,534 1 View

CMV and EF-1alpha promoter in same plasmid?

Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.

31 October 2023 9,919 2 View

ED50 of Meropenem in Mice against Klebsiella?

I am looking for the ED50 of meropenem in mice against Klebsiella systemic/lung infection models. Is anybody aware of it? Thank you.

18 June 2023 2,018 0 View

Which softwares are better to draw schematic diagrams of beams, plates etc?

I want to know which software is used by authors to draw schematic diagrams of beams, plates etc. for there articles.

22 May 2023 7,631 6 View

Is there a publication that discusses expression of different glycosylation enzymes in different CHO cell lines?

Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...

12 April 2023 9,492 2 View

DTM is the only method to solve elastic support beam vibration problem??

Most of the available literature related to vibration of nanobeam with elastic support boundary condition, author use Differential Transform Method (DTM) to solve the governing differential...

19 March 2023 3,763 2 View

Site directed mutagenesis not working. Can anyone help?

I am trying to replace a 20 bp region with a different sequence of same lengthin a 10kb plasmid. I designed overlapping primers. However after PCR I am only getting the template. At first I...

13 February 2023 3,663 3 View

Why does relative energy of conformers change when dispersion corrections are included during DFT calculations?

During conformational analysis of 18crown6, it was observed that the conformer with S6 symmetry was having the lowest energy when dispersion correction was not included. However, upon addition of...

14 February 2022 1,493 3 View

THP-1 requires differentiation before Phagocytosis assay?

I am looking to study opsonophagocytosis using THP-1 cell line from ATCC. Does the cell line require differentiation before the phagocytosis assay?

28 October 2021 3,412 5 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

HAs anyone used TGA to find activation enegy of water evaporation?

I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....

28 July 2024 9,545 6 View