Dear Friends,
I saw in many papers which the authors used cancerous cell line to check the toxicity of the small molecules by measuring the cell growth. I am wondering if using cancerous cell line in this application is a good choice? Because the compounds may not be toxic really and they may have anti-cancer effect.
Do you have any idea in checking toxicity of the compounds which one can be used? Cancerous cell line or normal cell lines?
Thanks,
Mohamad
it depends on your aim of experiment and the physiological relevance of the choice of cell lines
I am not sure 'normal cell lines' you mentioned are immortalised secondary cell lines or primary cell cause these two are very different by nature
Generally speaking, pros of using cancer cell line is that they are popular and easier to culture but like you implied, some cancer cells have very different physiology when compared to its primary cell equivalent. Immortalized non-cancer cell line are good alternative of cancer cell lines as they are somewhere closer to normal cells in vivo. primary cell lines or ex vivo culture are supposed to behave very similarly to cells in vivo but it takes high cost to maintain, in addition to high contamination risk. Primary cells could only be passed
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