Dear All,
According to atcc.org, SH-SY5Y cells have a reported saturation density greater than 1x10^6 cells/cm2. If that is true, one well of 6-well plate has surface area around 9.5 cm2, therefore at 100% confluence, it will have 9.5x10^6 cells of SH-SY5Y. In my newbie experiment with SH-SY5Y, 5x10^5 of SH-SY5Y is almost 70-80%confluence in 6-well. Do you have expirence with SH-SY5Y? What do you think?
Thank you
Cheerios,
Hi Asa. Interesting question..in my experience, SH-SY5Y never reach this kind of confluence..it depends on the fact that they are in reality composed by two different sub-species, and depending on the proportion between these two, they can reach confluence much earlier than this number...As a rule of thumb, I use 5-6X10E6 for each 100cm petri plate...
Dear Asa, this number corresponds to around 90-100% confluence and if you leave them for 1 replication cycle (around 36h) they reach stationary phase...and after that they grow quite slowly also if you subculture it....
Dear Fulvio, you mentioned that after the stationary phase they grow quite slowly also if you subculture it. Did you mean that when they reaches stationary phase, even I subculture (eg. divide ratio 1:20) them to new flask for next passage they will become slower growth compare to previous passage?
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