04 April 2016 10 2K Report

Dear All,

I tried to study co-localization of two different proteins by using immunofluorescence staining, since the western blot result of both protein shows significantly increase in nuclear extracts. However, both protein antibodies are rabbit host species, and I couldn't find other species antibody which has the same epiptope in both protein. I am afraid that changing the antibody can result different outcome.

Is there a way double-color immunofluorescence staining for two different protein, but have same rabbit host species antibodies?

Thank you very much

Cheerios,

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