Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View

How to input an exogenous variable in a GARCH model?

Hello, I have a question regarding financial time series. I am studying the effects of Quantitative Easing on the US stock market. I have tried to fit an ARIMA model to my S&P500 returns with...

01 February 2021 8,325 3 View

Kruskal-Wallis Test does not offer results in Model Viewer (SPSS 26.0)?

One of my students faces this problem in SPSS 26.0: When he runs Kruskal-Wallis test, the Output does not offer him access to the Model Viewer (where the pairwise comparison etc. would be...

11 November 2020 6,345 8 View

Can you recommend suitable primary antibody for Tau detection in rat?

Hi, I have problem detecting tau in rat primary astrocyte lysate by WB. I have used Tau46 form Santa Cruz Biotech (Sc-32274) but I don't see any band not even in positive control (rat brain...

23 May 2019 4,564 3 View

How to model utility grid in solar grid tied system in Simulink?

I am modelling grid tied solar PV with backup battery and I inserted load between solar system and grid. According to the graph of rms currents: Igrid = Ipv + Iload, which doesnt make any sense to...

10 May 2018 4,694 2 View

SPSS error "subscript is out of range", what to do?

21 January 2018 9,637 4 View

How to explain different patterns resulting after acridine orange assay for assessment of sperm DNA integrity?

We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...

02 October 2017 3,273 3 View

Method to make thin layer on glass substrate from nanopowder scintillator

Hi, does anyone have experience with making a thin film from scintillator nanopowder or nanopowder in general (GGAG:Ce, LuAG:Ce ... 10 - 30 nm particle size) on glass substrate. I writing master's...

14 August 2017 1,964 1 View

Does anyone experience with sonication of HCT116 on Bioruptor Pico?

17 July 2017 9,180 1 View

When MFI (mean fluorescence intensity) vs percent positive cells should be used?

I have analyzed platelet surface receptors using flow cytometry. The platelets were isolated from PRP, so, the samples contained only platelets. Every sample was stained with FITC-labeled...

01 February 2017 4,093 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View