Ondřej Šimoník

6 Questions 12 Answers 0 Followers

Questions related from Ondřej Šimoník

Is non-fluorescent antibody tag available?

Dear all, we would like to conjugate the primary antibody with a non-fluorescent tag for further analysis of labeled cells by light microscopy. Can anyone tell me if there is any possibility to...

19 June 2020 627 0 View

How to explain different patterns resulting after acridine orange assay for assessment of sperm DNA integrity?

We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...

03 October 2017 3,393 3 View

Does anybody use Yo-Pro 1 assay for apoptosis evaluation of stallion sperm cells?

Hello, please does anybody work with Yo-Pro 1/ PI protocol for staining apoptotic and necrotic sperms in stallion ejaculate? I have tried to use same protocol as for bovine sperms but it doesn't...

19 December 2014 4,589 4 View

Does anyone help me with problem concerning protocol YO-Pro1/PI, staining for apoptotic spermatozoa?

Hello, I would like ask somebody for infromation about protocol for apoptotic sperm cells staining, using Yo-Pro 1 probe and PI. I got protocol from one laboratory, where they use PI concentration...

10 October 2014 4,938 5 View

Has anyone ever used an Ice Cube 1810 freezer for embryos and spermatozoa and its LN2 supply?

Does anyone use this older type of freezer? I am wondering if I can use a "normal" container with LN2 adjusted by steel cap etc. as a source of nitrogen for freezer, or if it is necessary to buy a...

11 June 2014 2,103 2 View

Does anyone use propidium iodide for canine spermatozoa plasma membrane integrity assessment?

I am preparing for evaluation of canine ID's and I am wondering if I can use the same protocol for PI staining as for bull's sperms. Thank you kindly for any suggestions.

05 May 2014 1,477 9 View