10 Questions 17 Answers 0 Followers
Questions related from Ondřej Šimoník
Dear all, we would like to conjugate the primary antibody with a non-fluorescent tag for further analysis of labeled cells by light microscopy. Can anyone tell me if there is any possibility to...
19 June 2020 671 0 View
Dear all, we have tried for several times evaluation of motility (by CASA) and other functional parameters of sperm recovered from drops of media under mineral oil (Sigma, embryo tested). However...
10 October 2018 3,480 2 View
We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...
03 October 2017 3,471 3 View
Hello, could anyone suggest me proper concentration of calcium ionophore A23187 for induction of acrosome reaction of human sperm? Thank you in advance.
11 September 2017 8,085 5 View
I have started with evaluation of bull spermatozoa post-thaw with Flow cytometer. I have tried use PI/PSA protocol for simultaneous assessing viability and acrosomal integrity. After first FC...
12 August 2015 8,168 4 View
Hello, please does anybody work with Yo-Pro 1/ PI protocol for staining apoptotic and necrotic sperms in stallion ejaculate? I have tried to use same protocol as for bovine sperms but it doesn't...
19 December 2014 4,630 4 View
Hello, I would like ask somebody for infromation about protocol for apoptotic sperm cells staining, using Yo-Pro 1 probe and PI. I got protocol from one laboratory, where they use PI concentration...
10 October 2014 4,991 5 View
Does anyone use this older type of freezer? I am wondering if I can use a "normal" container with LN2 adjusted by steel cap etc. as a source of nitrogen for freezer, or if it is necessary to buy a...
11 June 2014 2,150 2 View
Does anyone use clarified egg yolk (centrifuged with re-destilled water) in a Tris canine semen extender? I am wondering if the added volume of this CEY is same as in case of whole egg yolk (20%)?...
15 May 2014 225 3 View
I am preparing for evaluation of canine ID's and I am wondering if I can use the same protocol for PI staining as for bull's sperms. Thank you kindly for any suggestions.
05 May 2014 1,526 9 View