Ondřej Šimoník

10 Questions 17 Answers 0 Followers

Questions related from Ondřej Šimoník

Is non-fluorescent antibody tag available?

Dear all, we would like to conjugate the primary antibody with a non-fluorescent tag for further analysis of labeled cells by light microscopy. Can anyone tell me if there is any possibility to...

19 June 2020 694 0 View

Does mineral oil affected sperm motility during in vitro analysis?

Dear all, we have tried for several times evaluation of motility (by CASA) and other functional parameters of sperm recovered from drops of media under mineral oil (Sigma, embryo tested). However...

10 October 2018 3,491 2 View

How to explain different patterns resulting after acridine orange assay for assessment of sperm DNA integrity?

We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...

03 October 2017 3,483 3 View

What is proper concentration of calcium ionophore A23187 for induction of acrosome reaction of human sperm?

Hello, could anyone suggest me proper concentration of calcium ionophore A23187 for induction of acrosome reaction of human sperm? Thank you in advance.

11 September 2017 8,091 5 View

Does anyone have experience with comparison of evaluation of sperm DNA integrity by flow cytometry using Propidium Iodide vs. SCSA ?

I have started with evaluation of bull spermatozoa post-thaw with Flow cytometer. I have tried use PI/PSA protocol for simultaneous assessing viability and acrosomal integrity. After first FC...

12 August 2015 8,172 4 View

Does anybody use Yo-Pro 1 assay for apoptosis evaluation of stallion sperm cells?

Hello, please does anybody work with Yo-Pro 1/ PI protocol for staining apoptotic and necrotic sperms in stallion ejaculate? I have tried to use same protocol as for bovine sperms but it doesn't...

19 December 2014 4,646 4 View

Does anyone help me with problem concerning protocol YO-Pro1/PI, staining for apoptotic spermatozoa?

Hello, I would like ask somebody for infromation about protocol for apoptotic sperm cells staining, using Yo-Pro 1 probe and PI. I got protocol from one laboratory, where they use PI concentration...

10 October 2014 5,001 5 View

Has anyone ever used an Ice Cube 1810 freezer for embryos and spermatozoa and its LN2 supply?

Does anyone use this older type of freezer? I am wondering if I can use a "normal" container with LN2 adjusted by steel cap etc. as a source of nitrogen for freezer, or if it is necessary to buy a...

11 June 2014 2,159 2 View

Has anyone added clarified egg yolk to canine semen?

Does anyone use clarified egg yolk (centrifuged with re-destilled water) in a Tris canine semen extender? I am wondering if the added volume of this CEY is same as in case of whole egg yolk (20%)?...

15 May 2014 230 3 View

Does anyone use propidium iodide for canine spermatozoa plasma membrane integrity assessment?

I am preparing for evaluation of canine ID's and I am wondering if I can use the same protocol for PI staining as for bull's sperms. Thank you kindly for any suggestions.

05 May 2014 1,534 9 View