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Questions related from Ondřej Šimoník
Dear all, we would like to conjugate the primary antibody with a non-fluorescent tag for further analysis of labeled cells by light microscopy. Can anyone tell me if there is any possibility to...
19 June 2020 629 0 View
We have performed AO assay on human ejaculated sperm. Everything seems, that works well, however we observed und microscope every cells stainned green and moreover there are two distinct...
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Hello, please does anybody work with Yo-Pro 1/ PI protocol for staining apoptotic and necrotic sperms in stallion ejaculate? I have tried to use same protocol as for bovine sperms but it doesn't...
19 December 2014 4,591 4 View
Hello, I would like ask somebody for infromation about protocol for apoptotic sperm cells staining, using Yo-Pro 1 probe and PI. I got protocol from one laboratory, where they use PI concentration...
10 October 2014 4,940 5 View
Does anyone use this older type of freezer? I am wondering if I can use a "normal" container with LN2 adjusted by steel cap etc. as a source of nitrogen for freezer, or if it is necessary to buy a...
11 June 2014 2,105 2 View
I am preparing for evaluation of canine ID's and I am wondering if I can use the same protocol for PI staining as for bull's sperms. Thank you kindly for any suggestions.
05 May 2014 1,478 9 View