Acquired from blood culture of a neonate child. Pinpoint colonies on blood agar, gamma haemolysis, urease positive, negative growth on SDA with chloramphenicol but growth + on Cornmeal agar at room temperature.
My work is to attenuate resistance gene which is adenine autotroph and i want to confirm mutant clone on M9 minimal media .recently I use 5 x M9 with 1 M glucose and adenine but it did not grow in...
03 March 2023 7,369 0 View
Hi! This is about protocol regarding antimicrobial susceptibility testing. I am from Asia and now doing my thesis. I am wondering whether i could find regional data and protocol regarding AST in...
17 January 2022 4,999 0 View
Electrochemical exfoliation of graphite was done using graphite electrodes in an acidic condition. After that, washing and drying of graphene oxide were done. Thereafter, reduction of graphene...
06 August 2020 8,178 3 View
Some systematic reviews reported NaF varnish can be applied topically two to four times a year. Is there any update about frequency of 2% NaF gel or varnish to apply in 6 years-old children? Can...
11 March 2019 5,473 5 View
My ongoing research is aimed to develop a product from my studies with field research. As we all knew that foodborne diseases are preventable but cause people unnecessary suffering. Among...
01 August 2017 4,592 4 View
Burma has adopted a Constitution in 2008, which features fake federal system. It has a long history of ethnic conflict between majority Burman and many other ethnic minority groups. The political...
24 September 2014 9,587 4 View
In mutation breeding work, we use PEG to screen the drought tolerance mutant in rice
28 January 2013 9,342 16 View
So, when I tried to id a Gram (-) bacilli from clinical spicmens. I tested oxidase disc test and sometimes, I ended up seeing oxidase positive and lactose ferment speices in many specimens. Most...
01 January 1970 5,121 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...
28 July 2024 6,824 0 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View