So, when I tried to id a Gram (-) bacilli from clinical spicmens. I tested oxidase disc test and sometimes, I ended up seeing oxidase positive and lactose ferment speices in many specimens. Most of the clinical specimens came from wound infection of pediatric and orthopedic patients. Does anyone encounter similar situations?
Yes. Sorry for my late reply.
I am using API 20 NE to ID such strain in my lab.
In fact, i am seeing many strongly oxidase positive GNB with TSI Acid/Acid profile.
In Table 7.1. First stage table for Gram Negative bacteria of Cowen & Steel, it clearly show that 7.9 Enterobacteria are oxidase negative.
For most commercially availabe semiauto and automatic bacteria Id kits and devices, the researchers may still need to sort out the genus of the target organisms.
For those scientsts working at well developed sequencing facilities, it would be nice to seqence, identify and report such interesting strains.
I think we do need to first check oxidase activity when we are identifying GNB now.
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