My colleague accidentally incubate fixative treated (Triton X-100, PFA, and DAPI) samples in CO2 incubator. All the fixing works were done in a biohood. Do you suggest autoclave the incubator? or Can I keep using it?
I agree with Daria. I would not be at all concerned about the DAPI or the triton. If the cells had been fixed in PFA and then rinsed to remove the fixative, the residue should be very very small. It shouldn't be a problem for using the incubator unless there was a very large number (>50) of samples that had been fixed in PFA. However I would avoid doing this in the future.
Hello dear,
It happened in our lab too and we ended up on the affect of PFA only in the plate which was having the same. Half wells were without fixative and to be used lateron for migration assay but incubating half of the wells in fixative for 15 mintues resulted in loss of migration and also cell rounding while it didn't have any affect on neighbouring cells.
So I will also suggest to use the same, what additionally can be advised is to open it's door fully (if the lab is quiet protected and sterile) and allow ventilation once for a while.
Hope it helps !
- Badges
- Science method