Chi Woo Yoon

10 Questions 2 Answers 0 Followers

Questions related from Chi Woo Yoon

Would physiological buffer solution be suitable for live cell imaging at 37 degree?

I have used different recipe of physiological buffer solutions for live cell imaging previously (such as live cell imaging solution from Thermofisher). Based on Thermofisher's description, " It...

12 December 2018 197 0 View

Real-time fluorescence imaging for insulin secretion?

I am looking for the way to measure insulin secretion in real-time from single cells using fluorescence microscope. Can anybody help me with this?

03 March 2017 8,593 2 View

Do you know any pharmaceutical agonist ATP-sensitivie potassium channels?

I am trying to block K_ATP dependent pathway for insulin secretion from pancreatic beta cells. Is there any pharmaceutical agents to specific for the pathway?

08 August 2016 3,628 2 View

How do you do glucose adaptation for pancreatic beta cell lines?

I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...

05 May 2016 9,877 2 View

KCl stimulation on breast cancer cells?

Has anyone tried KCl stimulation on human breast cancer cell lines such as MCF-7 or MDA-MB-231? or Has seen publication regarding that subject??

02 February 2016 8,267 1 View

How can I culture HIT-T15 in monolayer?

I am working with HIT-T15 pancreatic beta cell line.. In order to quantify, I need to count cells (when they are attached to culture dish) But it seemed that they grow in multiple layer even...

08 August 2015 8,188 2 View

How can I make fluorescence embedded gel for mechanical force measurement?

I am trying to fabricate gel with uniformly distributed fluorescence beads so that by measuring relative intensity of fluorescence signals, calculate 1. how much the gel is pushed 2. how much...

06 June 2015 3,153 3 View

Is it safe to use the incubator with cells treated with Triton x-100 and Paraformaldehyde ?

My colleague accidentally incubate fixative treated (Triton X-100, PFA, and DAPI) samples in CO2 incubator. All the fixing works were done in a biohood. Do you suggest autoclave the incubator? or...

02 February 2015 7,623 3 View

Can I keep plates with fixed cells in half of the wells and live cell in other half?

I am doing differentiation studies with MSC. I was inducing osteo- and adipo- differentiation in the same 24 well plate, and I want to stop the osteogenic differentiation and fix it for assay....

11 November 2014 7,738 5 View

How much HEPES should I add to differentiation inducing media for a couple hours long experiment outside the incubator?

I am working on human mesenchymal stem cells and trying to stimulate cells with high-frequency ultrasound microbeam. I want to observe cell morphology for a couple hours but we don't have a...

02 February 2014 9,414 1 View