Would physiological buffer solution be suitable for live cell imaging at 37 degree?

I have used different recipe of physiological buffer solutions for live cell imaging previously (such as live cell imaging solution from Thermofisher). Based on Thermofisher's description, " It...

11 December 2018 163 0 View

Real-time fluorescence imaging for insulin secretion?

02 March 2017 8,556 2 View

How do you do glucose adaptation for pancreatic beta cell lines?

I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...

04 May 2016 9,842 2 View

KCl stimulation on breast cancer cells?

Has anyone tried KCl stimulation on human breast cancer cell lines such as MCF-7 or MDA-MB-231? or Has seen publication regarding that subject??

01 February 2016 8,233 1 View

How can I culture HIT-T15 in monolayer?

I am working with HIT-T15 pancreatic beta cell line.. In order to quantify, I need to count cells (when they are attached to culture dish) But it seemed that they grow in multiple layer even...

07 August 2015 8,157 2 View

How can I make fluorescence embedded gel for mechanical force measurement?

05 June 2015 3,121 3 View

Is it safe to use the incubator with cells treated with Triton x-100 and Paraformaldehyde ?

My colleague accidentally incubate fixative treated (Triton X-100, PFA, and DAPI) samples in CO2 incubator. All the fixing works were done in a biohood. Do you suggest autoclave the incubator? or...

01 February 2015 7,584 3 View

Can I keep plates with fixed cells in half of the wells and live cell in other half?

10 November 2014 7,678 5 View

How much HEPES should I add to differentiation inducing media for a couple hours long experiment outside the incubator?

I am working on human mesenchymal stem cells and trying to stimulate cells with high-frequency ultrasound microbeam. I want to observe cell morphology for a couple hours but we don't have a...

01 February 2014 9,344 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Please, How can I plot a channel capacity of MIMO and SISO in matlab?

CSISO= f(frequency) : plot(f, CSISO) ? CMIMO= f(frequency) : plot (f, CMIMO) ?

13 February 2021 5,225 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The reason of PMMA channels cracking?

07 February 2021 3,663 2 View