Do you know any pharmaceutical agonist ATP-sensitivie potassium channels?

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Would physiological buffer solution be suitable for live cell imaging at 37 degree?

I have used different recipe of physiological buffer solutions for live cell imaging previously (such as live cell imaging solution from Thermofisher). Based on Thermofisher's description, " It...

11 December 2018 215 0 View

Real-time fluorescence imaging for insulin secretion?

I am looking for the way to measure insulin secretion in real-time from single cells using fluorescence microscope. Can anybody help me with this?

02 March 2017 8,608 2 View

How do you do glucose adaptation for pancreatic beta cell lines?

I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...

04 May 2016 9,890 2 View

KCl stimulation on breast cancer cells?

Has anyone tried KCl stimulation on human breast cancer cell lines such as MCF-7 or MDA-MB-231? or Has seen publication regarding that subject??

01 February 2016 8,280 1 View

How can I culture HIT-T15 in monolayer?

I am working with HIT-T15 pancreatic beta cell line.. In order to quantify, I need to count cells (when they are attached to culture dish) But it seemed that they grow in multiple layer even...

07 August 2015 8,201 2 View

How can I make fluorescence embedded gel for mechanical force measurement?

I am trying to fabricate gel with uniformly distributed fluorescence beads so that by measuring relative intensity of fluorescence signals, calculate 1. how much the gel is pushed 2. how much...

05 June 2015 3,169 3 View

Is it safe to use the incubator with cells treated with Triton x-100 and Paraformaldehyde ?

My colleague accidentally incubate fixative treated (Triton X-100, PFA, and DAPI) samples in CO2 incubator. All the fixing works were done in a biohood. Do you suggest autoclave the incubator? or...

01 February 2015 7,633 3 View

Can I keep plates with fixed cells in half of the wells and live cell in other half?

I am doing differentiation studies with MSC. I was inducing osteo- and adipo- differentiation in the same 24 well plate, and I want to stop the osteogenic differentiation and fix it for assay....

10 November 2014 7,757 5 View

How much HEPES should I add to differentiation inducing media for a couple hours long experiment outside the incubator?

I am working on human mesenchymal stem cells and trying to stimulate cells with high-frequency ultrasound microbeam. I want to observe cell morphology for a couple hours but we don't have a...

01 February 2014 9,438 1 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

What is the difference between alluvial and fluvial?

I have made an unpleasant discovery that I seemed to use these terms incorrectly. 'Alluvial' seems to mean "out of the river channel", flood-plain' etc. While all these cross-bedded...

15 July 2024 2,073 3 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How to add channel doping variation in sentaurus tcad to see its effect on transfer characteristics of mosfet?

I am getting trouble in studying the effect of channel doping variation in forksheet structure for p and nmos.

08 July 2024 2,074 0 View

What do you think are the key challenges that need to be addressed in the future development and application of these three devices?

Continuous glucose monitors (CGMs) help patients and doctors make more timely management decisions by monitoring blood glucose levels in real time. An insulin pump provides continuous insulin...

07 July 2024 7,742 0 View