Would physiological buffer solution be suitable for live cell imaging at 37 degree?

I have used different recipe of physiological buffer solutions for live cell imaging previously (such as live cell imaging solution from Thermofisher). Based on Thermofisher's description, " It...

11 December 2018 163 0 View

Real-time fluorescence imaging for insulin secretion?

02 March 2017 8,556 2 View

Do you know any pharmaceutical agonist ATP-sensitivie potassium channels?

I am trying to block K_ATP dependent pathway for insulin secretion from pancreatic beta cells. Is there any pharmaceutical agents to specific for the pathway?

07 August 2016 3,570 2 View

How do you do glucose adaptation for pancreatic beta cell lines?

I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...

04 May 2016 9,842 2 View

KCl stimulation on breast cancer cells?

Has anyone tried KCl stimulation on human breast cancer cell lines such as MCF-7 or MDA-MB-231? or Has seen publication regarding that subject??

01 February 2016 8,233 1 View

How can I culture HIT-T15 in monolayer?

I am working with HIT-T15 pancreatic beta cell line.. In order to quantify, I need to count cells (when they are attached to culture dish) But it seemed that they grow in multiple layer even...

07 August 2015 8,157 2 View

How can I make fluorescence embedded gel for mechanical force measurement?

05 June 2015 3,121 3 View

Is it safe to use the incubator with cells treated with Triton x-100 and Paraformaldehyde ?

My colleague accidentally incubate fixative treated (Triton X-100, PFA, and DAPI) samples in CO2 incubator. All the fixing works were done in a biohood. Do you suggest autoclave the incubator? or...

01 February 2015 7,584 3 View

Can I keep plates with fixed cells in half of the wells and live cell in other half?

10 November 2014 7,678 5 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View