I want better repeatability of my protocol and this is hindered by the fact I have to weight each phospholipids everytime i make a new batch which leads to slight changes and errors. So I would prefer to take out those lipids out of a solution instead. I will use the lipids dissolved in ethanol anyway so it would be really convenient.
These should be stable if stored in a freezer (-20oC) and flush the air out using N2 to reduce any oxidation if you have unsaturated fatty acids as components of your lipids. I sometimes use antioxidants (BHT) also if these do not interfer with your assays. Good luck
yes.It would be stable for 2 months provided that you should dissolve the recommended quantity /solubility ( 25-hydroxy cholesterol in ethanol and DMF is approximately 20 and 2 mg/ml) and remove the air out of the container with an inert gas.
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