Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

Which are the appropriate solvents for n-hexane plant extract for in vivo animal models to avoid toxicity?n-hexane plant extract is not soluble?

My n hexane plant extract is not soluble in any solvent. I have tried water, normal saline, tween 20 (2%, 5%, 10%, 50%), tween 80 (2%, 5%, 10%, 50%), pure DMSO, ethanol (2%, 5%, 10%, 50%, 97%),...

16 January 2021 3,547 3 View

What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

What are the primary defects associated with welding druring construction-stages which might influence the capacity of the gusset-plate connections??

Dear researchers What are the primary defects associated with welding druring construction-stages which might influence the capacity of the gusset-plate connections in concentrated braced frames?...

06 January 2021 797 3 View

Which are the Non Small Cell Lung Cancer markers?

I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...

04 January 2021 7,986 3 View

What is the new protocol followed by the World Health Organization to treat Covid 20?

The new strain of Covid 20 has spread in Britain, what is the new protocol for treating it, and is the old protocol followed by the World Health Organization suitable for treating the new strain...

21 December 2020 2,081 4 View

How can we convert powder (inorganic complex) in to a crystal?

I have powder (inorganic complex) and I want conversion it to crystal

03 December 2020 8,777 3 View

Can we use Google Form as our Research Tool?

I am confused regarding using Google Form as the questionaries tool for my study. It's effective during the covid-19 period but I didn't know if it is reliable or not. Please give your valuable...

18 November 2020 5,005 8 View

Can ELISA test be performed on whole blood?

Want to perform ELISA on whole blood instead of separating serum

03 October 2020 2,827 4 View

What is the difference between ultra-high vacuum chamber and edwards vacuum coating unit if we are using the the technique of thermal evaporation ?

developing thin films in ultra high vacuum chamber thermal evaporator.

09 September 2020 4,172 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Want to ask about equivalence ratio?

09 February 2021 5,273 2 View

Which kind of GPC solvent can be used to dissolve both lipid and salt?

I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).

14 January 2021 3,805 2 View

Would you tell me how to dissolve cholesterol in RPMI medium?

We are planning to add cholesterol to cells in order to raise the intracellular cholesterol concentration and confirm the protein expression of SREBP2. I didn't know if I should dissolve...

14 January 2021 871 6 View

How to remove ORO (Oil red O) background precipitates?

Hi ORO experts, I'm struggling with ORO background precipitates in arterial tissues. The protocol I use works very well on human atherosclerotic plaques which are full of lipids and I don't...

13 January 2021 7,110 1 View