Is it possible to select transgenic zebrafish/embryos by antibiotics (e.g. G418)?

Christoph Metzendorf @Christoph-Metzendorf

28 May 2020 0 3K Report

I tried to find out whether its feasible to use the SV40-promoter-NeoR cassette for antibiotic selection of transgenic fish. I was only able to find info about using G418 based selection of zebrafish cell culture cells but not of embryos. Has anyone ever tried? What concentrations of G418 have been used and during which developmental stages?

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