If yes, how many cells? in SC or into mammary fat pads? w/wo matrigel? estrogen supplementation required?
Dear Olivia,
I do not have a direct experience with this cells but they described in the literature as triple negative, so no estrogen supplementation should be needed for these cells to grow in xenograft models. In our lab, when we establish xenograft with MDA-MB-231 breast cancer cell, we transplant one million cells per mouse.
The transplantation site will depend on the type of study you want to conduct and it will also guide on the decision whether to use or not matrigel. If you implant sc, matrigel might create a more favourable environment for cells to engraft and give rise to tumor masses, while if you transplant orthotopically you should not need matrigel at all.
Good luck!
Matteo
Dear Olivia
I'm not sure you get the tumor because mice or rat may react against the human cell or the induced tumor shall be rejected in near future!
Dear Selvi,
Using immunocompetent mice for establishment of xenograft is not possible. Tumor cells will be cleared by the immune cells. The only reason I could think it might work is if the tumor cells have such a rapid and aggressive growth rate that the immune system is not able to kill them at the same rate. An option, if you think you really want to give it a try, is to irradiate recipient mice prior to transplantation, to slow down the immune response.
Good luck,
Matteo
Hi Olivia,
Were you able to establish tumors in mice with SUM52PE cells?
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