Hello everybody
my question is a methodological one: I would like to evaluate CXCL8 at intracellular levels. I have already evaluated CXCL8 levels in cells culture supernatants after 24 hours culture. My purpose is to understand if in those cells where CXCL8 is not secrete, it is maybe stored ad a protein at intracellular levels, thus if CXCL8mRNA is translated to a protein but not secreted in cell culture supernatant. I have no possibility to perform western blot, is it possible to assay it by ELISA? Could anyone suggest me the correct methodological approach?
Thankyou very much
Francesca
Hello Francesca Coperchini
You can use In-cell ELISA . Please refer to the link below for the protocol.
https://www.abcam.com/protocols/in-cell-elisa-ice
Hope this helps.
Best.
Francesca Coperchini
I suggest reading these articles first:
1. Article CXCL8/IL-8 and CXCL12/SDF-1α co-operatively promote invasive...
2. https://www.ncbi.nlm.nih.gov/gene/3576
3. https://www.nature.com/articles/s41592-021-01274-5
4. https://www.nature.com/articles/s41592-021-01274-5.pdf?origin=ppub
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