Is it possible to detect levels of VWF in cell culture supernatant by immunofloursence methods that are common in pathobiologic laboraatories?

16 June 2024 0 5K Report

Hi all, I mean chromogenic methods.

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Problems with designing syber-green primers for lnc_RNA GAS5?

Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?

15 June 2024 8,055 0 View

Symmetry Analysis in Abaqus-Hypermesh, How to manage?

Hello everyone I am trying to simulate a structure in Abaqus-Hypermesh insterface, considering the half of the model due to a symmetry. The model is in 3D and I'd like to know how can I acquire...

11 April 2024 1,868 0 View

What are major coagulant factors secreted from endothelial cells?

I found vWF, PAI1 and FVIII that are produced by endothelial cells, but I think there must be more, maybe more important factors to mediate coagulation process.

24 January 2024 6,165 3 View

Hello, how can I calculate the total amonia nitrogen (NH3+NH4) from NH4-N? that's because I used kit test for NH4-N but I need to know TAN?

Finally, I want to calculate free ammonium and for this purpose I need to know TAN.

29 October 2023 3,350 3 View

Is sum of two decreasing convergent sequence to 0, a decreasing convergent sequence to 0 in Archimedean Riesz space?

Is sum of two decreasing convergent sequence to 0, a decreasing convergent sequence to 0 in Archimedean Riesz space? If an and bn are o-convergent, is an+bn also o-convergent?

01 September 2022 4,093 2 View

Grey cast iron defect with graphite nodule around the hole?

we are casting grey cast Iron with DIZA machine. recently we have faced a new defect as a surface hole and the hole is surrounded by a thin layer of nodular graphite. the pictures from the...

31 August 2021 2,922 6 View

What is the most suitable NMR solvent for carbon-based materials?

25 December 2020 5,267 4 View

Effect of adsorbent porosity on efficiency of adsorbent recovery ?

Hi guys. Can anyone answer the following with citing the references? 1) Relationship between adsorbent porosity and adsorption/desorption rate? 2)Relationship between porosity and adsorbent...

31 October 2020 6,537 5 View

Where is the best location for placement of a magnetic filter in a amine system?

One solution which is presented for eliminating suspended Iron sulfide (FeS) contamination from refinery amine system, is using from magnetic separation. where location of amine circulation (rich...

09 September 2020 10,071 6 View

What is teaching? rethinking about concept of teaching after coronavirus?

every person knows that teaching is the most important weapon we can use change the world. but there is an important question which education ? or teaching? when I think this question my mind...

04 August 2020 7,184 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View