Ghasem Ajam

6 Questions 2 Answers 0 Followers

Questions related from Ghasem Ajam

Stimulating cell culture with bacteria?

How can I stimulate a normal cell line with G- bacteria? I need a standard protocol.

02 February 2025 7,920 0 View

Problems with designing syber-green primers for lnc_RNA GAS5?

Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?

16 June 2024 8,099 0 View

Is it possible to detect levels of VWF in cell culture supernatant by immunofloursence methods that are common in pathobiologic laboraatories?

Hi all, I mean chromogenic methods.

16 June 2024 4,711 0 View

Which part of lncRNAs is more suitable to design primers for?

Hi, It seems lncRNAs have not exon parts on NCBI gene bank file, so if I want to design primers to detect for example MALAT1 by qRT-PCR (syber-green) in cell culture extract, which section is...

14 March 2024 2,923 1 View

What are major coagulant factors secreted from endothelial cells?

I found vWF, PAI1 and FVIII that are produced by endothelial cells, but I think there must be more, maybe more important factors to mediate coagulation process.

25 January 2024 6,202 3 View

Where can I find ELISA Kit for CCHF?

Hello I live in Iran and this kind of kits are not available for us here, Could someone tell me how can I find if?

26 September 2023 4,804 1 View