How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can I remove salts from crude seaweed extract (except dialysis)?

Hi, I am interested to obtain phenolic fractions from crude seaweed extracts (using ethanol) and test for their antioxidant properties. Since most of the in-vitro antioxidant assays are...

26 January 2021 3,889 5 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Do you know any practical application of alginate extraction from seaweed? Can you give me an example?

Do you know any practical application of alginate extraction from seaweed? Can you give me an example?

01 December 2020 808 3 View

Is there a natural conservant to increase shelf life of seaweed liquid extract? Sugarcane molasses could be used?

We are looking for methods to increase lifeshelf of seaweed extracts to be used as liquid fertilizer which can maintain the organic carachteristic of the product. Does anyone has expertise in this...

12 November 2020 1,570 2 View

How to remove water from plant extract??

Hello everyone, I hope these words find you well. I used 80% methanol (solvent) for seaweed's extraction. I have performed rotary evaporator extraction in order to evaporate methanol but there is...

16 October 2020 5,598 8 View

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro (laboratory)culture?

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have...

10 August 2020 302 3 View

Is there any pcr primers for amplifying seaweed specific rbcL gene from environmental samples?

For eDNA barcoding, is there any primer published that proved to pick seaweed rbcL genes from eDNA samples? A universal primer that could amplify rbcL from Rhodophyta, chlorophyta and Ochrophyta...

10 July 2020 9,566 3 View

What Solvent is appropriate for DPPH when extracting with ethanol?

I am working with seaweeds. I am using using ethanol as my extraction solvent based on preliminary experiment and using methanol to dissolve my DPPH, should I expect any difference than, when I...

09 July 2020 1,052 14 View

How much seaweed biomass to use in a pot of soil?

Hi, I am having trouble in researching how much seaweed to use in a pot of 500mg soil as most papers address the use of seaweed extract. Any recommendations on how much seaweed to mix with soil...

01 February 2020 7,714 1 View