Actually, I am trying to culture seaweeds inside lab for experimental purpose. I'm facing contamination in spore culture and also I can't get a proper growth response with juvenile algae. I m using commercial white fluorescent light, cotton filtered autoclave seawater, pH 7.8 with PES media in 5litre closed containers which having aeration from upside. I have a doubt that I need to do a cultures with opened containers or closed completely. Anyone pls tell me

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